Low Binding Microtubes CLEARLock CLEARLine

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Low Binding Microtubes CLEARLock CLEARLine

Low Binding Microtubes CLEARLock™ CLEARLine®

Sample loss during handling is a critical issue for the success of the analysis, especially when volumes are small, concentrations are low
or processing steps are extensive.

To prevent potential interactions between analytes and tubes, Biosigma has created the new Low Binding CLEARLock™ CLEARLine® Microtubes line.

The new Microtubes are designed to minimize protein and nucleic acid loss without using any surface coating such as silicone. Biosigma
Low-bind material ensures optimal sample recovery and reproducible results, reducing the risk of interference with the samples.
The Low Binding Protein and DNA Microtubes are ideal for both sample preparation and long-term storage, especially for low
concentration samples that require minimal protein and DNA loss.

Each batch is certified free from human DNA, DNase, RNase, ATP, endotoxins, and PCR inhibitors.

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Product Information

Protein recovery with Low Binding Protein Microtubes CLEARLock™ CLEARLine® compared with other suppliers.

Test procedure:

Low Binding protein CLEARLock ™ CLEARLine ® Microtubes from Biosigma, suppliers 1 and 2 (Low binding and standard) were filled with an aqueous BSA solution and human protein extracts in a concentration of 40ng/ml and stored at 4°C for 24hrs.
After the incubation, proteins were extracted, concentration determined by Bradford assay, values normalized for Biosigma microtubes recovery and used to generate the following graphic.
3 independent experiments were performed.
Average recovery >20% compared to standard tubes.

DNA recovery with Low Binding DNA Microtubes CLEARLock™ CLEARLine® compared with other suppliers.

Test procedure:

Low Binding DNA CLEARLock™ CLEARLine® Microtubes from Biosigma, suppliers 1 and 2 (Low binding and standard) were filled with 3,5ng/µl and 0,35ng/µl of Human Nuclear DNA and stored at -80°C for 24hrs.
After the incubation, 5 and 10 µl of DNA solution were amplified by PCR (35 and 40 cycles), the intensities of bands were calculated using ImageJ, values normalized for Biosigma Microtubes amplified products, and used to generate the following graphic.
3 independent experiments were performed.
Average recovery >20% compared to standard tubes.

Features

MAIN CHARACTERISTICS:

◾ Maximum Nucleic Acid and Protein recovery rates
◾ Hinged lid prevents any unintentional opening during use
◾ Precise lid sealing avoids evaporation during long-term storage
◾ Pierceable writing cap
◾ Graduated with writing area
◾ Optimal transparency
◾ Centrifugation up to 30,000 x g
◾ Temperature range: -80°C + 100°C
◾ Autoclavable at +121°C for 20 minutes
◾ Zip bags, re-closable
◾ Particularly suitable for Molecular Biology and Biochemistry
◾ In Polypropylene Medical Grade, Non Cytotoxic – Non Hemolytic
◾ Recommended for chemical and byological sample storage
◾ Human DNA, DNase, RNase, Endotoxin, ATP, PCR inhibitors free certified*