CALCULATION METHOD FOR CELLS / µL IN URINARY SEDIMENT
FAST READ 102® is a disposable plastic device composed of 10 counting chambers. Each device allow to analyze 10 samples.
Each room contains a GRID composed of 10 SQUARES divided into 16 smaller squares (called SECTORS).
One advantage of using FAST-READ is to determine easily the specimen’s number of cells per µL
1. Perform the count on a centrifuged fresh urine sample, after decantation
2. Gently resuspend the sediment
3. Using a pipette, introduce the sample into the well and examine under the microscope the area of the grid
4. Count the number of cellular elements within N squares
Cell. / µl = (Σ cells counted in square N) x concentration factor x 10
Concentration factor = Volume of sediment / Volume centrifuged urine
10: conversion from 0.1µl to 1µl
For uncentrifuged urine, don’t multiply the number of cells counted
For the concentration factor.
METHOD OF CELL COUNTS FOR DILUTED SAMPLES (CELLS / ML)
After filling the counting chamber with the sample, proceed with the
counting of the cells distributed in N squares.
Considering that the grid consists of 10 squares, each square has a
Dimensions of 1 x 1 mm, a depth of 0.1 mm and a volume of 0.1 µl,
the formula for determining the concentration of cells (cells / ml ) is:
Cells / ml = (Σ cells counted in square N) x dilution factor x 104
104 = conversion 0.1 µl in 1 ml
Pay attention to the cells on the edges, you should only count those
on either side, to avoid over or under counting risk.
In the example showns, we perform the cell counting of a sample
diluted 100 times:
N = 5 (number of squares considered for counting) cells counted in
5 square = 67
Dilution factor = 102
[Cells / ml] = (67/5) x 102 x 104 = 13.4 x 106
This product fulfils the requirements of Directive 98/79/EC on in vitro diagnostic medical devices